Taken together with other cell biological and genetic studies during the past several years, it seems fair to conclude that condensins are the most important, central players in the assembly and structural maintenance of mitotic chromosomes ( Thadani et al., 2012 Frosi and Haering, 2015 Hirano, 2016 Kalitsis et al., 2017). A recent study has used the same cell-free extracts to demonstrate that chromosome-like structures can be assembled even in the near absence of nucleosomes ( Shintomi et al., 2017). Moreover, once assembly reactions are complete, removing topo IIα from mitotic chromosomes has little impact on their morphology in the Xenopus egg cell-free extracts ( Hirano and Mitchison, 1993). Equally important, a recent study has demonstrated that mitotic chromosome-like structures can be reconstituted in vitro by mixing sperm chromatin with only six purified components, which include core histones, topo IIα, and condensin I ( Shintomi et al., 2015). In fact, only two factors, topoisomerase IIα (topo IIα) and condensin I, have been demonstrated so far to be essential for mitotic chromatid assembly in the cell-free extracts ( Hirano and Mitchison, 1993 Hirano et al., 1997). It is therefore possible that the “core” components required for building the bulk part of mitotic chromosomes is much simpler, as had been shown in classical studies of metaphase chromosomes isolated from HeLa cells ( Gasser and Laemmli, 1987) or mitotic chromatids assembled in Xenopus egg cell-free extracts ( Hirano and Mitchison, 1994). It should be noted, however, that this number includes domain-specific components (e.g., centromere- and telomere-specific proteins) and contaminants that may artificially get associated with chromosomes during their isolation. In fact, a recent proteomics approach has identified ∼4000 proteins in mitotic chromosomes isolated from chicken DT40 cells ( Ohta et al., 2010). It is generally thought that the protein composition of mitotic chromosomes is highly complex, especially because they represent one of the largest structures observed within the cell. Despite enormous progress marked during the past two decades or so, its molecular mechanism remains not fully understood ( Belmont, 2006 Marko, 2008 Kinoshita and Hirano, 2017). This process, known as mitotic chromosome assembly or condensation, is an essential prerequisite for faithful segregation of genetic information into two daughter cells. When eukaryotic cells divide, chromatin residing within the interphase nucleus is converted into a discrete set of individual chromosomes, each composed of a pair of rod-shaped chromatids (sister chromatids). We propose that condensin II makes a primary contribution to mitotic chromosome architecture and maintenance in human cells. Furthermore, quantitative morphological analyses using the machine-learning algorithm wndchrm support the notion that chromosome shaping is tightly coupled to the reorganization of condensin II-based axes. This assay combined with small interfering RNA depletion demonstrates that the recovery of chromatin shapes and the reorganization of axes are highly sensitive to depletion of condensin II but less sensitive to depletion of condensin I or topoisomerase IIα. Both chromatin and condensin-positive chromosome axes are converted into near-original shapes at 100 mM NaCl. In this assay, mitotic chromosomes are first expanded in a hypotonic buffer containing a Mg 2+-chelating agent and then converted into different shapes in a NaCl concentration-dependent manner. To address these questions from a physico-chemical point of view, we have established a set of two-step protocols for inducing reversible assembly of chromosome structure in situ, namely within a whole cell. Although both complexes become concentrated along the axial region of each chromatid by metaphase, it remains unclear exactly how such axes might assemble and contribute to chromosome shaping. Condensins I and II are multisubunit complexes that play a central role in mitotic chromosome assembly.
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“We’re taking a road not taken so there may be low hanging fruit, that nobody else picked because it was not taken,” Loeb said.įor all that UFO research may be becoming destigmatized, Loeb said the field is still looked down upon by some astrophysicists and other academics, which can turn off young scientists. A computer will use artificial intelligence to analyze the data, ignoring objects like birds, drones, planes, and meteors, and paying extra attention to any objects “that are not human-made”, Loeb said. The telescope will use infra-red cameras to take 24/7 video of the sky, and is equipped with a radio sensor, an audio sensor and a magnetometer to detect non-visual objects. Loeb plans to make the projects’ findings publicly available. The privately funded project, which involves more than 100 scientists, is building its first telescope system on the roof of the Harvard college observatory, and it will begin operating this summer. He is the head of the Galileo Project, which aims to establish a network of sophisticated telescopes which will scan the skies for extraterrestrial objects.Ĭlosed gates at the entrance to Area 51, the military base in Nevada. Still, as the clamor for information has grown, so has interest from the scientific community, and in 2022 a slew of new projects will launch, specifically aimed at detecting alien life.Īvi Loeb, the Frank B Baird Jr professor of science at Harvard University, is behind one of those. Since then the Pentagon, pushed by US senators Kirsten Gillibrand and Marco Rubio, has launched a new office for reporting and analyzing UFO reports, although some in the UFO community suspect the government to be less than forthcoming with its findings. Officials were able to explain one of the incidents – it was a balloon – but the rest remain a mystery. The Pentagon studied 144 incidents reported by military pilots between 20 in preparing the report. The US government’s UFO report, released in June 2021, fueled more interest. The account of the navy pilots was given credibility, however, by leaked military footage which showed an oval flying object near a US navy ship off San Diego, and separate videos which showed triangular-shaped objects buzzing around in the sky. “Every day for at least a couple years.”įor years, pilots had refused to share tales of their UFO experiences, worried of being labeled kooks or being passed over for promotion. It happened so frequently that the encounters became commonplace, Ryan Graves, a retired navy pilot, told the CBS show. In a breakthrough interview with 60 Minutes, members of the US Navy lined up to recall their experiences of encountering UFOs on America’s coasts. It was a group of pilots who brought the issue to the fore in 2021. “Finally, I think we’ll see more high-calibre witnesses coming forward, including commercial airline pilots, military aircrew, radar operators, and intelligence officers with direct knowledge of this subject.” Some of this may come via whistleblowers, but much of it may be released by the government itself, either proactively, or in response to requests under the Freedom of Information Act. “I also think we’ll see the release of more US military photos and videos of UFOs, and associated documents. “I think we’ll see congressional hearings on UFOs,” Pope said. In Congress, where a bipartisan group of senators has been pushing for years for the government to release more information on UFOs, and from the US defense department and intelligence community, Pope said he senses “a genuine desire to grip the issue”. “I’m confident that 2022 is going to be a seismic year for UFOs,” said Nick Pope, who spent the early 1990s investigating UFOs for the British ministry of defence. The report came after leaked military footage documented seemingly otherworldly happenings in the sky, and after testimony from navy pilots helped to somewhat destigmatize a subject that has long been defined by conspiracy theories and dubious sightings.Īll in all, the newly sincere approach to UFOs has longtime sky-watchers excited. In June, the Pentagon released a highly anticipated report on unexplained aerial phenomena (UAP), the now preferred nomenclature by some in the extraterrestrial community, which found more than 140 instances of UAPs that could not be explained. In Homestuck^2: Beyond Canon (Dubiously Canon) In a development image from when Chittr was to be called "Prongle", users' handles seem to be referred to as trolltags. In Hiveswap Chittr Xefros “ xXstickit2meXx “ dammarchy ” John and Jane's Dads go by “ pipefan 413 ”, and the Draconian Dignitary simply goes by “ The Dignitary ”, with various other human and carapacian users of Serious Business going by even more varied and strange handles.ĭave uses the same handle on Delirious Biznasty as he does on Pesterchum, with the official Crockercorp account going by “ Bett圜rocker ”. Handles on Serious Business and Delirious Biznasty show far more variety than those used on the kids' chat clients, and are only abbreviated in particularly long cases such as that of the Condesce, who uses Serious Business as her full title “ )(er Imperious Condescension ” shortened to “ )(IC ”. Instead of the letters G, C, A or T, the cherubs Calliope and Caliborn have their handles abbreviated instead with the letter U, which is used in place of the letter T when describing molecules of RNA.ĭ oc Scratch does not appear to have a handle, instead appearing as ?_? when in contact with Aradia, ? when in contact with Kanaya (or Karkat), and ? when talking to Gamzee. While Jane ( “ GG ”) and Jake ( “ GT ”) both follow this pattern also, the rest of the alpha humans do not however each of their handle abbreviations matches with that of one of their children. Aradia ( “ AA ”) and Feferi ( “ CC ”) also follow this pattern, but the rest of the troll girls have unique two-letter abbreviations instead. Interestingly, as noted by Andrew Hussie, the abbreviations of the beta human handles correlate with the chromosome pairs traditionally associated with the characters' gender identities when they were introduced to the comic “ TT ” and “ GG ” are like-letters, like XX, whereas the letters in “ GT ” and “ TG ” differ as in XY. The one exception to this rule is John Egbert, who did at one time have a handle abbreviated to GT but later changed it. The human and troll heroes all have handles whose abbreviations consist of the letters G, C, A, and T, symbols commonly used to represent parts of a DNA molecule the humans' handles use only G and T, whereas the trolls use all four letters. All known users of these clients use two-word handles, which the clients represent in camel case and abbreviate into two capital letters (with the exception of Caliborn, whose handle is abbreviated in lowercase instead to match his quirk), but is unknown if this is a strict requirement. Pesterchum and Trollian both make use of chat handles, where they are called chumhandles and trolltags, as does BettyBother. 3 In Homestuck^2: Beyond Canon (Dubiously Canon). Note: If the tracks have not been modified, then the action which should be described by relationships is compilation, not DJ mixing. To describe a 'DJ mix', use the DJ mix relationships. A DJ mix requires that the tracks be modified in some manner. Common techniques for this include crossfading, beat-matching, beat juggling or scratching. DJ mixĪ sequence of several songs played one after the other, each one modified so that they blend together into a continuous flow of music. To describe a 'compilation', use the Compiler relationships. A compilation requires only that the tracks be played one after another. Mix terminology compilationĮither an entire album, or one long track which contains multiple songs played one after the other. The interface for editing a release's relationships, available via the "Edit relationships" link.This is used to mean two different things: See the special purpose track title guidelines and, where necessary, the guidelines for multiple titles for details about how to name these tracks. one or more songs that follow another song on the same track, usually after a longer period of silence, but are not listed on the sleeve. is located before the first track (a so-called pregap track). a song in the pregap of track 1 on a CD, i.e.a track that is not listed on the release's sleeve.MusicBrainz identifier Glossary hidden track.4.14 remastering Basic MusicBrainz entities.The album even has the band’s founder crooning like a demented Dean Martin on the track “Over and Out.” An obvious listen for hardcore NIN fans, but a single spin should suffice for everyone else. But then, Bad Witch devolves into an almost Zorn-like, avant-jazzy journey into frenzied, dissonant noise that, at times, is almost too grating to the ear. Interestingly, the song “34 Ghosts IV” was recently sampled by Lil Nas X in his chart-topping hit, “ Old Town Road.” The result? A CMA nomination for Reznor.Īt first, this final EP in a trilogy shows signs of promise: “Shit Mirror” leaps right out of the gate, replete with thrashy guitars, bombastic rhythms, and Reznor’s signature angsty vocals, followed by “Ahead of Ourselves,” a track that harkens back to Nine Inch Nails of yore. If nothing else, it was voluminous, with nearly 40 songs released under the Ghosts banner, and provided fans with a preview of what was to come from Reznor, who, at that time, revealed he was turning his focus to film scoring. The largely instrumental, atmospheric collection followed his bitter divorce from Interscope Records and was mostly improvised, reportedly over two-plus months in the studio. In what was a rather prolific year for Reznor, Ghosts I-IV proved that not all experiments he undertakes work. Where to start? Well, the first nine songs were released for free for a reason, folks. Without further ado, here is our painstaking assessment of Reznor’s best-kicking off, of course, with the worst of the bunch. With two of Nine Inch Nails’ records reaching milestone anniversaries this year ( The Fragile is 20, and Pretty Hate Machine 30), we figured what better time to take a look back at Reznor’s output of major releases? A nearly impossible task, yes-but someone had to do it.Īlso Read Danny Elfman Reimagines Bigger. Each song has conveyed his pain and torment, all intertwined with introspective lyrics-some so deeply personal, they almost feel invasive to listen to. Over the course of three productive decades, Reznor has delivered uncompromising and simultaneously poignant albums. Almost immediately, the Thomas Edison of industrial set himself apart from his contemporaries-including Ministry and Front 242-unleashing emotive, penetrating tunes that worked both in the clubs and in the pits. Often imitated but never replicated, Nine Inch Nails seemingly came out of nowhere in the late 1980s and hit like an earthquake, right around the commencement of hair metal’s unceremonious funeral procession. It’s tantamount to calling Kim Jong-un a “bad dude” or Dwayne “The Rock” Johnson an “actor.” The fact is, there has never before been a musician quite like the industrial-rock nihilist. It would be a blatant oversimplification to label Nine Inch Nails mastermind Trent Reznor a musical genius. If you’re a serious hat geek looking for the ins and outs of deep-cut cap minutiae, that’s not what this book is about. Interesting, right? Colby’s daily cap project during the pandemic reminded me of my own Pandemic Porch Cocktails photo project (itself a collection of sorts) - many people told me how they sort of bonded with that project in 2020 and ’21, just as people apparently bonded with Colby’s cap ritual. I was wearing a part of my life every day. There are fun, sad, uplifting, universal stories about life, death, love, loss and triumph. So is the relationship with my brothers and parents. So is the trip to NYC to take his sons to Yankee Stadium, something Dave told me he wanted to do in one of the last emails I received from him. Meeting my best friend, Dave, is in there. Also, I realized that I was actually writing about all the connections in my life. My mother is 87 and I know she really wants to see me publish a book. My cousin Julie, who writes mysteries, and my mother, who writes for the local paper, posted that I should write a book. Legitimate bonds had been created by sharing hats. He also said I’m the best friend he’s ever had who he’d never met. Jim told me it had helped him get through the pandemic. A few people told me they were sad it was over. When I made my last post, people who had never even marked a “like” on any of my posts started telling me how much they enjoyed the daily hat and story. I have never met Jim in person, but he wanted to keep the connection going.Īfter 125 days, I ran out of hats. One gentleman, Jim, a Viet Nam veteran who lives in Texas, ran out of hats about 25 days in, but he didn’t want to miss out on the fun, so he started sharing whatever he could find - autographs, pictures, CDs, bobbleheads. A little community quickly formed around the posts, sharing our lives through our love of hats. I started adding little stories with the hats. The first was how long we’d be locked down. Every day, I posted a picture on Facebook of the hat I’d wear. And there’s an interesting backstory, which Colby explains like so:Ī week into the lockdown in March 2020, I decided to wear a different hat every day until we were allowed to be together again. I’m not a cap collector myself, but I’ve collected lots of other things over the years, so I can relate to the story-driven approach Colby’s taking here - it’s a good concept. (There’s a short scene from the 1987 movie Throw Momma From the Train that illustrates this point really well.) That’s the idea behind a new book by Craig Colby, called All Caps: Stories That Justify an Outrageous Hat Collection. But often they’re also collections of stories. Open the Instagram app and allow it to load, you want to be sure that the person’s story you want to view is showing in your app, so it loads.Some collections are just collections of objects.Here’s how to see someone’s story on Instagram without them knowing, by turning off your internet connection: When you do this, it doesn’t register on Instagram that you’ve viewed the story because you viewed it while offline. One of the best ways to view someone’s Instagram story without them knowing is to do it with your internet off. And if you want to unsee an Instagram DM, read about how to read Instagram messages without being seen. Here is another useful tutorial for Instagram stories: Add Multiple Photos Or Longer Videos To Instagram Stories. So, you stand a risk of losing a follower in case they don’t follow you afterward.ĭon’t Miss: How To Add Temperature In Instagram Story Follow the user again if you still want to follow them.Īfter that story has expired, you will have to follow them (if you were following them earlier) again as blocking someone removes them from your following.Īnother thing you should know is that blocking someone will make them unfollow you (if they were following you earlier). Due to the steep walls, the Halos has an effective length of 220 cm. Both of the doors on the inner tent are D-shaped, and the mosquito net can be fully covered. Our Wechsel Groundsheets gives your tent extra protection from wet grounds, thorns, sharp rocks and moisture. The inner tent has 12 pockets to stash stuff. Research Popular Providers near Helotes, Texas. 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Buy Wechsel Halos 3 Groundsheet at Bergzeit Online Huge selection Free delivery on orders from 50 Outdoor experts since 1999. One of the intersections is higher than the others, which allows rainwater to drain off and provides exceptional stability against the wind. 8034 Halo Cir, San Antonio, TX is a single family home that contains 2,241 sq ft and was built in 2017. The Groundsheet is made of a robust 75D polyester with a PU coating on the inside (7000mm water column). Tent Ground sheets & Footprints by Wechsel Huge brand selection - Free. The three poles cross at the upper roof area. That means that you can put up the whole tent at once (the inner tent is hung inside). Wechsel - Halos 3 - 3-man tent Dispatch within 24h - Buy online now 100 Day Return Policy Expert advice Free. 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I have mentioned in my reviews of “The Sixteen Millimeter Shrine” in regards to the main character, and of “Escape Clause” in regards to the main character’s wife, we want to see bad characters come to bad endings and good or innocent characters come to happy endings. “That’s just not fair There was time now.” “That’s not fair,” he says as tears begin to fall. He soliloquizes in happiness, until, reaching to pick up another book, his glasses fall off and he can no longer see. Even a life as the only man on a destroyed earth was a life worth living, if it had books. He finds undamaged books, and, at the steps of the library, realizes that he’s finally found everything he ever wanted. He reasons that he’d be forgiven, given the circumstances, and is about to shoot himself, when he spots a library. Now officially completely alone, he wanders in despair, eventually finding a gun. He goes to his old home, and finds that, like everything else, it’s destroyed. They look great in black and white, and the shots of Bemis wandering around awestruck are unforgettable. Bemis finds his glasses and heads out to see the total destruction as promised by the newspaper. He reads a newspaper, whose headline reads “H-Bomb Capable of Total Destruction.” This is our first indication of anything of the sort, and it works, because the story is ultimately about the character, and it sucks you in that way. One day, Bemis goes down into the bank vault to read during his lunch break in peace and quiet. It’s such a horrendous action that it really makes you feel for the character, and chances are you liked him already, even if you found him ridiculous. In a scene with his wife, she tricks him into thinking she wants to hear some poetry, only to allow him to discover that she has crossed out the words on every single page of his book. When getting scolded by his boss, Bemis confesses that his wife won’t let him read at home, and that’s why he reads at work. There are a few funny lines, and Meredith plays the role perfectly awkward. These opening scenes are played as light comedy, and they work as that. We see early on that his constant reading prevents him from being good at his job, and gets him in trouble with his boss. It features perhaps the series’s strongest character and performance, and has an unforgettable ending.īurgess Meredith ( Of Mice and Men, Rocky) plays Henry Bemis, a myopic bookish little bank teller that seems to get on everyone’s nerves. This episode seems to always be in the running in people’s minds for best ever. Episode 1.8 “Time Enough at Last Original air date: NovemWriter: Rod Serling (short story by Lynn Venable) Director: John Brahm Solid ONE PIECE roto-molded quality kayak, loa. Ideal for year round use on lakes and easy rivers like the metro ‘Hooch. Better than new with several enhancements.
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